Anti-Crkl Antibody (6J402) is a Rabbit antibody targeting Crkl. Anti-Crkl Antibody (6J402) can be used in WB,ICC/IF,IP,FCM.

IgG

6J402

Human,Mouse,Rat

1. 25 ug total protein per lane of various lysates (see on figure) probed with Crkl monoclonal antibody, unconjugated (TMAB-04681) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The K562 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-Crkl antibody (TMAB-04681, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
3. 4% Paraformaldehyde-fixed K562 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Crkl) monoclonal Antibody, unconjugated (TMAB-04681) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

WB: 1:500-2000; ICC/IF: 1:50-200; IP: 1:20-50; FCM: 1:50-100

Monoclonal

A synthesized peptide: human CRKL

Human

CRKL

Crk-like protein

May mediate the transduction of intracellular signals.