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Anti-CRISP10 Polyclonal Antibody is a Rabbit antibody targeting CRISP10. Anti-CRISP10 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
| Description | Anti-CRISP10 Polyclonal Antibody is a Rabbit antibody targeting CRISP10. Anti-CRISP10 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Sample: Heart (Mouse) Lysate at 40 μg Primary: Anti-CRISP10 (TMAB-04674) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD 2. Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CRISP10) Polyclonal Antibody, Unconjugated (TMAB-04674) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Human stomach cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CRISP10) Polyclonal Antibody, Unconjugated (TMAB-04674) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Cysteine-rich secretory proteins (CRISPs) represent a family of evolutionarily conserved proteins which may play a role in the innate immune system and are transcriptionally regulated by androgens in several tissues. CRISP proteins are highly expressed in the mammalian reproductive tract and in the venom secretory ducts of some reptiles. CRISP-10 (cysteine-rich secretory protein 10), also known as CocoaCrisp and Trypsin inhibitor Hl, is a 500 amino acid protein containing 2 LCCL domains, which are thought to function as autonomous folding domains used to construct modular proteins through exon shuffling. CRISP-10 differs from other CRISP proteins in that it does not contain the 10 conserved cysteine residues or ICR domains that are usually conserved throughout the CRISP family. |
| Immunogen | KLH conjugated synthetic peptide: human CRISP10 |
| Antigen Species | Human |
| Gene Name | CRISPLD1 |
| Gene ID | |
| Protein Name | Cysteine-rich secretory protein LCCL domain-containing 1 |
| Uniprot ID |
| Molecular Weight | Theoretical: 54 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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