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Anti-CRHR1 Polyclonal Antibody
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Catalog No. TMAB-00481
Alias Seven transmembrane helix receptor, Releasing hormone receptor 1, MGC124240, MGC124237, CRHR1L, CRH-R1h, CRHR1f, CRH-R-1, CRH-R1, CRHR 1, CRHR, CRH R1h, CRF-RI, CRF-R-1, CRF-R1, CRFR1, CRF-R, CRF1, CRF R, CRF 1, Corticotropin releasing hormone receptor variant 1h, corticotropin releasing hormone receptor 1
Anti-CRHR1 Polyclonal Antibody is a Rabbit antibody targeting CRHR1. Anti-CRHR1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-CRHR1 Polyclonal Antibody
Anti-CRHR1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00481Alias Seven transmembrane helix receptor, Releasing hormone receptor 1, MGC124240, MGC124237, CRHR1L, CRH-R1h, CRHR1f, CRH-R-1, CRH-R1, CRHR 1, CRHR, CRH R1h, CRF-RI, CRF-R-1, CRF-R1, CRFR1, CRF-R, CRF1, CRF R, CRF 1, Corticotropin releasing hormone receptor variant 1h, corticotropin releasing hormone receptor 1
Anti-CRHR1 Polyclonal Antibody is a Rabbit antibody targeting CRHR1. Anti-CRHR1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CRHR1 Polyclonal Antibody is a Rabbit antibody targeting CRHR1. Anti-CRHR1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | Seven transmembrane helix receptor, Releasing hormone receptor 1, MGC124240, MGC124237, CRHR1L, CRH-R1h, CRHR1f, CRH-R-1, CRH-R1, CRHR 1, CRHR, CRH R1h, CRF-RI, CRF-R-1, CRF-R1, CRFR1, CRF-R, CRF1, CRF R, CRF 1, Corticotropin releasing hormone receptor variant 1h, corticotropin releasing hormone receptor 1 |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human) |
| Verified Activity | 1. Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 μg Lane 2: Cerebellum (Mouse) Lysate at 40 μg Lane 3: Testis (Mouse) Lysate at 40 μg Lane 4: Cerebrum (Rat) Lysate at 40 μg Lane 5: Cerebellum (Rat) Lysate at 40 μg Primary: Anti-CRHR1 (TMAB-00481) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50-55 kDa Observed band size: 48 kDa 2. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CRHR1) Polyclonal Antibody, Unconjugated (TMAB-00481) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CRHR1) Polyclonal Antibody, Unconjugated (TMAB-00481) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Tissue/cell: Rat adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-CRHR1 Polyclonal Antibody, Unconjugated (TMAB-00481) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants one of which is a non-coding read-through transcript with the neighboring gene MGC57346. |
| Immunogen | KLH conjugated synthetic peptide: human CRHR1 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cancer,Obesity,Hypothalamic pituitary adrenal axis,Metabolism,Corticoid |
| Molecular Weight | Theoretical: 48 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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