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Anti-CRHR1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00481
Alias Seven transmembrane helix receptor, Releasing hormone receptor 1, MGC124240, MGC124237, CRHR1L, CRH-R1h, CRHR1f, CRH-R-1, CRH-R1, CRHR 1, CRHR, CRH R1h, CRF-RI, CRF-R-1, CRF-R1, CRFR1, CRF-R, CRF1, CRF R, CRF 1, Corticotropin releasing hormone receptor variant 1h, corticotropin releasing hormone receptor 1

Anti-CRHR1 Polyclonal Antibody is a Rabbit antibody targeting CRHR1. Anti-CRHR1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-CRHR1 Polyclonal Antibody

Anti-CRHR1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00481Alias Seven transmembrane helix receptor, Releasing hormone receptor 1, MGC124240, MGC124237, CRHR1L, CRH-R1h, CRHR1f, CRH-R-1, CRH-R1, CRHR 1, CRHR, CRH R1h, CRF-RI, CRF-R-1, CRF-R1, CRFR1, CRF-R, CRF1, CRF R, CRF 1, Corticotropin releasing hormone receptor variant 1h, corticotropin releasing hormone receptor 1
Anti-CRHR1 Polyclonal Antibody is a Rabbit antibody targeting CRHR1. Anti-CRHR1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3747-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-CRHR1 Polyclonal Antibody is a Rabbit antibody targeting CRHR1. Anti-CRHR1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasSeven transmembrane helix receptor, Releasing hormone receptor 1, MGC124240, MGC124237, CRHR1L, CRH-R1h, CRHR1f, CRH-R-1, CRH-R1, CRHR 1, CRHR, CRH R1h, CRF-RI, CRF-R-1, CRF-R1, CRFR1, CRF-R, CRF1, CRF R, CRF 1, Corticotropin releasing hormone receptor variant 1h, corticotropin releasing hormone receptor 1
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human)
Verified Activity
1. Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 μg
Lane 2: Cerebellum (Mouse) Lysate at 40 μg
Lane 3: Testis (Mouse) Lysate at 40 μg
Lane 4: Cerebrum (Rat) Lysate at 40 μg
Lane 5: Cerebellum (Rat) Lysate at 40 μg
Primary: Anti-CRHR1 (TMAB-00481) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50-55 kDa
Observed band size: 48 kDa
2. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CRHR1) Polyclonal Antibody, Unconjugated (TMAB-00481) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CRHR1) Polyclonal Antibody, Unconjugated (TMAB-00481) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Tissue/cell: Rat adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CRHR1 Polyclonal Antibody, Unconjugated (TMAB-00481) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants one of which is a non-coding read-through transcript with the neighboring gene MGC57346.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CRHR1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Cancer,Obesity,Hypothalamic pituitary adrenal axis,Metabolism,Corticoid
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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