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Anti-CREB5 Polyclonal Antibody
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Catalog No. TMAB-00480
Alias Cyclic AMP-responsive element-binding protein 5, CRE-BPa, CREB-5, Creb5, cAMP-responsive element-binding protein 5
Anti-CREB5 Polyclonal Antibody is a Rabbit antibody targeting CREB5. Anti-CREB5 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
Anti-CREB5 Polyclonal Antibody
Anti-CREB5 Polyclonal Antibody
😃Good
Catalog No. TMAB-00480Alias Cyclic AMP-responsive element-binding protein 5, CRE-BPa, CREB-5, Creb5, cAMP-responsive element-binding protein 5
Anti-CREB5 Polyclonal Antibody is a Rabbit antibody targeting CREB5. Anti-CREB5 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CREB5 Polyclonal Antibody is a Rabbit antibody targeting CREB5. Anti-CREB5 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB. |
| Synonyms | Cyclic AMP-responsive element-binding protein 5, CRE-BPa, CREB-5, Creb5, cAMP-responsive element-binding protein 5 |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Chicken,Dog,Cow,Horse) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CREB5) Polyclonal Antibody, Unconjugated (TMAB-00480) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: Human HeLa cell lysates Lane 2: Human HepG2 cell lysates Lane 3: Human MCF-7 cell lysates Primary: Anti-CREB5 (TMAB-00480) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kDa Observed band size: 60 kDa 3. Paraformaldehyde-fixed, paraffin embedded (human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CREB5) Polyclonal Antibody, Unconjugated (TMAB-00480) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.    |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | The product of this gene belongs to the CRE (cAMP response element)-binding protein family. Members of this family contain zinc-finger and bZIP DNA-binding domains. The encoded protein specifically binds to CRE as a homodimer or a heterodimer with c-Jun or CRE-BP1, and functions as a CRE-dependent trans-activator. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human CREB5 |
| Antigen Species | Human |
| Gene Name | CREB5 |
| Gene ID | |
| Protein Name | Cyclic AMP-responsive element-binding protein 5 |
| Uniprot ID | |
| Biology Area | Zinc Finger |
| Function | Binds to the cAMP response element and activates transcription. |
| Molecular Weight | Theoretical: 57 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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