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Anti-CREB5 Polyclonal Antibody

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Catalog No. TMAB-00480

Anti-CREB5 Polyclonal Antibody is a Rabbit antibody targeting CREB5. Anti-CREB5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-CREB5 Polyclonal Antibody

Anti-CREB5 Polyclonal Antibody

😃Good
Catalog No. TMAB-00480
Anti-CREB5 Polyclonal Antibody is a Rabbit antibody targeting CREB5. Anti-CREB5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$373 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF

Reactivity: Human,Mouse (predicted:Rat,Chicken,Dog,Cow,Horse)
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Chicken,Dog,Cow,Horse)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CREB5) Polyclonal Antibody, Unconjugated (TMAB-00480) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human HepG2 cell lysates
Lane 3: Human MCF-7 cell lysates
Primary: Anti-CREB5 (TMAB-00480) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 60 kDa
3. Paraformaldehyde-fixed, paraffin embedded (human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CREB5) Polyclonal Antibody, Unconjugated (TMAB-00480) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe product of this gene belongs to the CRE (cAMP response element)-binding protein family. Members of this family contain zinc-finger and bZIP DNA-binding domains. The encoded protein specifically binds to CRE as a homodimer or a heterodimer with c-Jun or CRE-BP1, and functions as a CRE-dependent trans-activator. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CREB5
Antigen Species
Human
Gene ID
9586
Uniprot ID
Biology Area
Zinc Finger
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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