Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF

Reactivity: Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Sheep)

IgG

Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Sheep)

1. Sample:
293t (Human) Cell Lysate at 30 μg
Primary: Anti-CREB-1 (TMAB-00479) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
2. Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CREB-1 Polyclonal Antibody, Unconjugated (TMAB-00479) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample: Hela Cell Lysate at 40 μg
Primary: Anti-CREB-1 (TMAB-00479) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit Igg (secondary antibody) at 1: 5000 dilution;
Predicted band size:37 kDa
Observed band size:37 kDa
4. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CREB-1) Polyclonal Antibody, Unconjugated (TMAB-00479) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CREB-1

Human

1385

HLH / Leucine Zipper,Transcription Factors,TLR Signaling