Anti-CREB-1 Polyclonal Antibody is a Rabbit antibody targeting CREB-1. Anti-CREB-1 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse,Rat

1. Sample:
Lane 1: Cerebrum (Rat) Lysate at 40 μg
Lane 2: Kidney (Mouse) Lysate at 40 μg
Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Lane 4: Jurkat (Human) Cell Lysate at 30 μg
Primary: Anti-CREB-1 (TMAB-04650) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 47 kD
2. Blank control (blue line): Hela cells (fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃).
Primary Antibody (green line): Rabbit Anti-phospho-CREB-1 antibody (TMAB-04650),
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 μg /test.
3. Sample: A549 (Human) Lysate at 30 μg
Primary: Anti-CREB-1 (TMAB-04650) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD
4. Sample: U251 (Human) Lysate at 30 μg
Primary: Anti-CREB-1 (TMAB-04650) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human CREB-1

Human

CREB1

Cyclic AMP-responsive element-binding protein 1

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.