Anti-COX1/MTCO1 Polyclonal Antibody is a Rabbit antibody targeting COX1/MTCO1. Anti-COX1/MTCO1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Mouse,Rat (predicted:Human,Chicken,Dog,Pig,Cow,Horse,Rabbit)

1. Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (COX1) Polyclonal Antibody, Unconjugated (TMAB-00468) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Sample:
Lane 1: Mouse Heart Lysates
Lane 2: Mouse Skeletal muscle Lysates
Lane 3: Rat Heart Lysates
Lane 4: Rat Muscle Lysates
Primary: Anti-COX1MTCO1 (TMAB-00468) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 45 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human COX1

Human

MT-CO1

Cytochrome c oxidase subunit 1

Integration of energy metabolism,Integration of energy,Mitochondrial markers,Complex IV,Cancer,Mitochondrial,Mitochondria

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.