Anti-CORO7 Polyclonal Antibody is a Rabbit antibody targeting CORO7. Anti-CORO7 Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF.

IgG

Human (predicted:Mouse,Rat,Dog,Cow,Horse,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (Human hepatocellular carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CORO7) Polyclonal Antibody, Unconjugated (TMAB-04591) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CORO7) Polyclonal Antibody, Unconjugated (TMAB-04591) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
3. MDA-MB-231 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (CORO7) polyclonal Antibody, Unconjugated (TMAB-04591) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CORO7

Human

CORO7

Coronin-7

May play a role in the maintenance of the Golgi apparatus morphology and in the protein export from the Golgi.