Anti-COPE Antibody (1J185) is a Mouse antibody targeting COPE. Anti-COPE Antibody (1J185) can be used in WB,ICC/IF.

IgG1, Kappa

1J185

Human

1. Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1: 20000
Primary Ab incubation condition: room temperature 2 h
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: HeLa, 2: 293, 3: MCF-7
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 32 kDa
Observed MW: 32 kDa
2. Cell line: HeLa
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-04570

WB: 1:2000-5000; ICC/IF: 1:100-500

Monoclonal

COPE

Coatomer subunit epsilon

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.