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Anti-Complement C3 Antibody (7T917) is a Rabbit antibody targeting Complement C3. Anti-Complement C3 Antibody (7T917) can be used in FCM,ICC/IF,WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $297 | 7-10 days | |
100 μL | $498 | 7-10 days |
Description | Anti-Complement C3 Antibody (7T917) is a Rabbit antibody targeting Complement C3. Anti-Complement C3 Antibody (7T917) can be used in FCM,ICC/IF,WB. |
Synonyms | Complement Component C3a, C3a, Anaphylatoxin |
Ig Type | IgG |
Clone | 7T917 |
Reactivity | Human |
Verified Activity | 1. ICC staining C3 in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 2. Flow cytometric analysis of HepG2 cells with C3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody 3. Western blot analysis of C3 / C3a expression in Human plasma lysate. 4. All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature. |
Application | |
Recommended Dose | WB: 1:1000-2000; ICC/IF: 1:50-200; FCM 1:20-100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
Research Background | C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. |
Conjucates | Unconjugated |
Immunogen | A synthesized peptide: human Complement C3 |
Antigen Species | human |
Uniprot ID |
Molecular Weight | Theoretical: 187 kDa. |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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