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Anti-Complement C3 Antibody (7T917)

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Catalog No. TMAC-00504
Alias Complement Component C3a, C3a, Anaphylatoxin

Anti-Complement C3 Antibody (7T917) is a Rabbit antibody targeting Complement C3. Anti-Complement C3 Antibody (7T917) can be used in FCM,ICC/IF,WB.

Anti-Complement C3 Antibody (7T917)

Anti-Complement C3 Antibody (7T917)

😃Good
Catalog No. TMAC-00504Alias Complement Component C3a, C3a, Anaphylatoxin
Anti-Complement C3 Antibody (7T917) is a Rabbit antibody targeting Complement C3. Anti-Complement C3 Antibody (7T917) can be used in FCM,ICC/IF,WB.
Pack SizePriceAvailabilityQuantity
50 μL$2977-10 days
100 μL$4987-10 days
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Product Introduction

Bioactivity
Description
Anti-Complement C3 Antibody (7T917) is a Rabbit antibody targeting Complement C3. Anti-Complement C3 Antibody (7T917) can be used in FCM,ICC/IF,WB.
SynonymsComplement Component C3a, C3a, Anaphylatoxin
Ig Type
IgG
Clone
7T917
Reactivity
Human
Verified Activity
1. ICC staining C3 in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
2. Flow cytometric analysis of HepG2 cells with C3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
3. Western blot analysis of C3 / C3a expression in Human plasma lysate.
4. All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Application
Recommended Dose
WB: 1:1000-2000; ICC/IF: 1:50-200; FCM 1:20-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Research BackgroundC3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthesized peptide: human Complement C3
Antigen Species
human
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 187 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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