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Anti-Collagen X/COL10A1 Polyclonal Antibody is a Rabbit antibody targeting Collagen X/COL10A1. Anti-Collagen X/COL10A1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
| Description | Anti-Collagen X/COL10A1 Polyclonal Antibody is a Rabbit antibody targeting Collagen X/COL10A1. Anti-Collagen X/COL10A1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P. |
| Synonyms | wu:fb10c08, wu:fa66d11, Schmid metaphyseal chondrodysplasia, Procollagen type X alpha 1, OTTHUMP00000040411, fb10c08, fa66d11, CollagenX, collagen X, alpha-1 polypeptide, Collagen X alpha 1 polypeptide, Collagen type X alpha 1(Schmid metaphyseal chondrodysplasia), Collagen type X alpha 1, Collagen type X, collagen alpha-1(X) chain precursor, Collagen alpha-1(X) chain, Collagen alpha 1(X) chain, COL10A1, Col10a 1, COAA1 |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Chicken,Dog,Cow,Rabbit,Pig) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Collagen X) Polyclonal Antibody, Unconjugated (TMAB-00463) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Collagen X) Polyclonal Antibody, Unconjugated (TMAB-00463) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Collagen X) Polyclonal Antibody, Unconjugated (TMAB-00463) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. ![]() ![]() ![]() |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix. |
| Tissue Specificity | Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | This gene encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD). [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human Collagen X |
| Antigen Species | Human |
| Gene Name | COL10A1 |
| Gene ID | |
| Protein Name | Collagen alpha-1(X) chain |
| Uniprot ID | |
| Biology Area | Collagen |
| Function | Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage. |
| Molecular Weight | Theoretical: 73 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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