Anti-Collagen VI Antibody (4T333) is a Rabbit antibody targeting Collagen VI. Anti-Collagen VI Antibody (4T333) can be used in IHC-P,IHC-Fr,IF,WB.

IgG

4T333

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Collagen VI Monoclonal Antibody, Unconjugated (TMAB-04527) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Collagen VI Monoclonal Antibody, Unconjugated (TMAB-04527) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Collagen VI Monoclonal Antibody, Unconjugated (TMAB-04527) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. 25 μg total protein per lane of various lysates (see on figure) probed with Collagen VI monoclonal antibody, unconjugated (TMAB-04527) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
5. Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen VI Monoclonal Antibody, Unconjugated (TMAB-04527) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen VI Monoclonal Antibody, Unconjugated (TMAB-04527) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen VI Monoclonal Antibody, Unconjugated (TMAB-04527) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen VI Monoclonal Antibody, Unconjugated (TMAB-04527) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen VI Monoclonal Antibody, Unconjugated (TMAB-04527) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Mouse Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen VI Monoclonal Antibody, Unconjugated (TMAB-04527) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen VI Monoclonal Antibody, Unconjugated (TMAB-04527) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen VI Monoclonal Antibody, Unconjugated (TMAB-04527) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen VI Monoclonal Antibody, Unconjugated (TMAB-04527) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.

IHC-P: 1:200-800; IHC-Fr: 1:200-800; IF: 1:200-800; WB: 1:1000-5000

Monoclonal

A synthesized peptide: human Collagen VI

Human

COL6A1

Collagen alpha-1(VI) chain

Collagen VI acts as a cell-binding protein.