Anti-Collagen III Antibody (5Y376) is a Mouse antibody targeting Collagen III. Anti-Collagen III Antibody (5Y376) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

5Y376

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Monoclonal Antibody, Unconjugated (TMAB-04523) at 1:1000 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Monoclonal Antibody, Unconjugated (TMAB-04523) at 1:1000 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Monoclonal Antibody, Unconjugated (TMAB-04523) at 1:1000 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Monoclonal Antibody, Unconjugated (TMAB-04523) at 1:1000 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Abdominal skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Monoclonal Antibody, Unconjugated (TMAB-04523) at 1:1000 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Monoclonal Antibody, Unconjugated (TMAB-04523) at 1:1000 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Monoclonal Antibody, Unconjugated (TMAB-04523) at 1:1000 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Monoclonal Antibody, Unconjugated (TMAB-04523) at 1:1000 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
9. 25 μg total protein per lane of various lysates (see on figure) probed with Collagen III monoclonal antibody, unconjugated (TMAB-04523) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
10. 4% Paraformaldehyde-fixed RD (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Collagen III) monoclonal Antibody, unconjugated (TMAB-04523) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

WB: 1:500-2000; IHC-P: 1:500-1000; IHC-Fr: 1:500-1000; ICC/IF: 1:100-200; IF: 1:500-1000

Monoclonal

Recombinant Protein: human Collagen III protein

Human

COL3A1

Collagen alpha-1(III) chain

Collagen type III occurs in most soft connective tissues along with type I collagen.