Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF

Reactivity: Human,Mouse (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit)

IgG

Human,Mouse (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit)

1. Sample:
293t (Human) Cell Lysate at 30 μg
A549 (Human) Cell Lysate at 30 μg
LOVO (Human) Cell Lysate at 40 μg
MCF-7 (Human) Cell Lysate at 30 μg
HepG2 (Human) Cell Lysate at 30 μg
Primary: Anti-Collagen II (TMAB-00458) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDa
2. Sample:
MG63 (Human) Cell Lysate at 30 μg
Primary: Anti-Collagen II (TMAB-00458) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDa
3. Paraformaldehyde-fixed, paraffin embedded (mouse ear); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Collagen II) Polyclonal Antibody, Unconjugated (TMAB-00458) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
4. Tissue/cell: Mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Collagen II Polyclonal Antibody, Unconjugated (TMAB-00458) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
5. Sample: bone (Mouse) Lysate at 40 μg
Primary: Anti-Collagen Ii (TMAB-00458) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 117 kDa
Observed band size: 134 kDa

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Collagen II

Human

1280