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Anti-Collagen II/COL2A1 Polyclonal Antibody 2
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Catalog No. TMAB-00458
Alias STL1, SEDC, collagen, type II, α1, collagen, type II, alpha 1, COL11A3, AOM, ANFH
Anti-Collagen II/COL2A1 Polyclonal Antibody 2 is a Rabbit antibody targeting Collagen II/COL2A1. Anti-Collagen II/COL2A1 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Collagen II/COL2A1 Polyclonal Antibody 2
Anti-Collagen II/COL2A1 Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00458Alias STL1, SEDC, collagen, type II, α1, collagen, type II, alpha 1, COL11A3, AOM, ANFH
Anti-Collagen II/COL2A1 Polyclonal Antibody 2 is a Rabbit antibody targeting Collagen II/COL2A1. Anti-Collagen II/COL2A1 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Collagen II/COL2A1 Polyclonal Antibody 2 is a Rabbit antibody targeting Collagen II/COL2A1. Anti-Collagen II/COL2A1 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | STL1, SEDC, collagen, type II, α1, collagen, type II, alpha 1, COL11A3, AOM, ANFH |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Sample: 293t (Human) Cell Lysate at 30 μg A549 (Human) Cell Lysate at 30 μg LOVO (Human) Cell Lysate at 40 μg MCF-7 (Human) Cell Lysate at 30 μg HepG2 (Human) Cell Lysate at 30 μg Primary: Anti-Collagen II (TMAB-00458) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 117 kDa Observed band size: 117 kDa 2. Sample: MG63 (Human) Cell Lysate at 30 μg Primary: Anti-Collagen II (TMAB-00458) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 117 kDa Observed band size: 117 kDa 3. Paraformaldehyde-fixed, paraffin embedded (mouse ear); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Collagen II) Polyclonal Antibody, Unconjugated (TMAB-00458) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 4. Tissue/cell: Mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Collagen II Polyclonal Antibody, Unconjugated (TMAB-00458) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 5. Sample: bone (Mouse) Lysate at 40 μg Primary: Anti-Collagen Ii (TMAB-00458) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 117 kDa Observed band size: 134 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human Collagen II |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID |
| Molecular Weight | Theoretical: 117 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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