Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,ICC/IF,IF

Reactivity: Human,Mouse,Rat

IgG

Human,Mouse,Rat

1. 25 μg total protein per Lane of various lysates probed with Collagen I polyclonal antibody, unconjugated (TMAB-00455) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
2. U-118 MG (H) cells were treated with or without BFA, 25 μg total protein per Lane of cell lysates probed with Collagen I polyclonal antibody, unconjugated (TMAB-00455) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
14. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
15. Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
16. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
17. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
18. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
19. Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
20. 4% Paraformaldehyde-fixed U-87MG (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (Collagen I) polyclonal Antibody, unconjugated (TMAB-00455) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.
21. The U-87MG (H) cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C,the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-Collagen I antibody (TMAB-00455,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test.Blank control (black): PBS.
22. The U2-OS (H) cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C,the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-Collagen I antibody (TMAB-00455,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Blank control (black): PBS.
23. 4% Paraformaldehyde-fixed U2-OS (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (Collagen I) polyclonal Antibody, unconjugated (TMAB-00455) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.

WB,IHC-P,IHC-Fr,ICC/IF,IF

WB: 1:200-1000; IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; ICC/IF: 1:50-200; IF: 1:200-1000

Polyclonal

KLH conjugated synthetic peptide: human Collagen I

Human

1277

Collagen,Osteogenesis