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Anti-Collagen I Antibody (9B199)
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Catalog No. TMAB-04519
Anti-Collagen I Antibody (9B199) is a Mouse antibody targeting Collagen I. Anti-Collagen I Antibody (9B199) can be used in IHC-P,IHC-Fr,IF.
Anti-Collagen I Antibody (9B199)
Anti-Collagen I Antibody (9B199)
Copy Product Info😃Good
Catalog No. TMAB-04519
Anti-Collagen I Antibody (9B199) is a Mouse antibody targeting Collagen I. Anti-Collagen I Antibody (9B199) can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 200 μg | $1,057 | 7-10 days | 7-10 days | |
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Bioactivity
Antigen Details
Chemical Properties
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| Description | Anti-Collagen I Antibody (9B199) is a Mouse antibody targeting Collagen I. Anti-Collagen I Antibody (9B199) can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Clone | 9B199 |
| Reactivity | Human |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (TMAB-04519) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (TMAB-04519) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (TMAB-04519) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (TMAB-04519) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (TMAB-04519) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix. |
| Tissue Specificity | Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. |
| Immunogen | KLH conjugated synthetic peptide: human Collagen I |
| Antigen Species | Human |
| Gene Name | COL1A1 |
| Gene ID | |
| Protein Name | Collagen alpha-1(I) chain |
| Uniprot ID | |
| Function | Type I collagen is a member of group I collagen (fibrillar forming collagen). |
| Molecular Weight | Theoretical: 139 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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