Anti-COL5A1 Polyclonal Antibody is a Rabbit antibody targeting COL5A1. Anti-COL5A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Rabbit,Sheep)

1. Tissue/cell: rat skin tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Collagen V Polyclonal Antibody, Unconjugated (TMAB-04516) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-GM-CSF Polyclonal Antibody, Unconjugated (TMAB-04516) 1: 200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated used at 1: 200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml, blue) was used to stain the cell nuclei
3. Sample:
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Skin tissue lysates
Lane 3: Mouse Uterus tissue lysates
Lane 4: Rat Uterus tissue lysates
Lane 5: Human A549 cell lysates
Primary: Anti-COL5A1 (TMAB-04516) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 153 kDa
Observed band size: 200 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human COL5A1

Human

COL5A1

Collagen alpha-1(V) chain

Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.