Anti-Cofilin Polyclonal Antibody is a Rabbit antibody targeting Cofilin. Anti-Cofilin Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Dog,Pig,Cow,Sheep)

Blank control (Black line): Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-cofilin antibody (TMAB-04511)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 1μg/Test

Polyclonal

Synthetic peptide from the human cofilin conjugated to KLH

Human

CFL1

Cofilin-1

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization.