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Anti-CNTF Polyclonal Antibody
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Catalog No. TMAB-04499
Anti-CNTF Polyclonal Antibody is a Rabbit antibody targeting CNTF. Anti-CNTF Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-CNTF Polyclonal Antibody
Anti-CNTF Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-04499
Anti-CNTF Polyclonal Antibody is a Rabbit antibody targeting CNTF. Anti-CNTF Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Antigen Details
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| Description | Anti-CNTF Polyclonal Antibody is a Rabbit antibody targeting CNTF. Anti-CNTF Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Rat,Rabbit (predicted:Human,Mouse,Pig) |
| Verified Activity | 1. Tissue/cell: rat spinal cord tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-CNTF Polyclonal Antibody, Unconjugated (TMAB-04499) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-CNTF Polyclonal Antibody, Unconjugated (TMAB-04499) 1: 200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1: 200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml, blue) was used to stain the cell nuclei 3. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-CNTF Polyclonal Antibody, Unconjugated (TMAB-04499) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 4. Tissue/cell: rabbit brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-CNTF Polyclonal Antibody, Unconjugated (TMAB-04499) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Tissue Specificity | Nervous system. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. In addition to the predominant monocistronic transcript originating from this locus, the gene is also transcribed as a readthrough transcript with the upstream Zfp91 gene. The resulting protein is an isoform of Zfp91 and does not include Cntf sequence. Readthrough transcription of Zfp91 and Cntf has been observed in human and mouse. |
| Immunogen | KLH conjugated synthetic peptide: human CNTF |
| Antigen Species | Human |
| Gene Name | CNTF |
| Gene ID | |
| Protein Name | Ciliary neurotrophic factor |
| Uniprot ID | |
| Function | CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. |
| Molecular Weight | Theoretical: 23 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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