Anti-CNPase Polyclonal Antibody is a Rabbit antibody targeting CNPase. Anti-CNPase Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Dog,Cow,Horse,Rabbit)

1. Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CNP/CNPase Polyclonal Antibody, Unconjugated (TMAB-04494) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CNP/CNPase Polyclonal Antibody, Unconjugated (TMAB-04494) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CNPase) Polyclonal Antibody, Unconjugated (TMAB-04494) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
4. Sample: Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-CNPase (TMAB-04494) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution
Predicted band size: 48 kD
Observed band size: 46 kD
5. Sample: Brain (Mouse) Lysate at 40 μg
Primary: Anti-CNPase (TMAB-04494) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CNPase

Human

CNP

2', 3'-cyclic-nucleotide 3'-phosphodiesterase

May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin.