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Anti-CNN1 Antibody (5Y848) is a Rabbit antibody targeting CNN1. Anti-CNN1 Antibody (5Y848) can be used in WB,ICC/IF,IHC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $297 | 7-10 days | |
100 μL | $497 | 7-10 days |
Description | Anti-CNN1 Antibody (5Y848) is a Rabbit antibody targeting CNN1. Anti-CNN1 Antibody (5Y848) can be used in WB,ICC/IF,IHC. |
Synonyms | smooth muscle, SMCC, Sm Calp, HEL S 14, Epididymis secretory protein Li 14, CNN 1, Calponins basic, Calponin-1, Calponin1, Calponin H1 smooth muscle, Calponin H1, Calponin 1 basic smooth muscle, Basic calponin |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat,Dog,Pig |
Verified Activity | 1. Western blot analysis of Calponin on Hela cell lysates using anti-Calponin antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Calponin antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti-Calponin antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Calponin antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Calponin antibody. Counter stained with hematoxylin. 6. ICC staining Calponin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Application | |
Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | Calponin regulates smooth muscle cell contraction and is a marker of smooth muscle cell differentiation. Calponin, an Actin- and Tropomyosin-binding protein, is characterized as an inhibitory factor of smooth-muscle actomyosin activity. Calponin is implicated in the regulation of smooth muscle contraction through its interaction with F-Actin and inhibition of the Actin-activated MgATPase activity of phosphorylated Myosin. Both properties are lost following phosphorylation (primarily at Serine 175) by protein kinase C or calmodulin-dependent protein kinase II. The three forms of Calponin, Calponin 1 (basic Calponin), Calponin 2 (neutral Calponin) and Calponin 3 (acidic Calponin), are found in smooth muscle tissue. Additionally, Calponin 2 is found in heart muscle tissue and Calponin 3 is found in the brain. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 33 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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