Anti-CNKSR2/KSR2 Polyclonal Antibody is a Rabbit antibody targeting CNKSR2/KSR2. Anti-CNKSR2/KSR2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Rabbit)

1. Sample:
U251 (Human) Cell Lysate at 30 μg
Primary: Anti-CNKSR2/KSR2 (TMAB-04487) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 114 kD
Observed band size: 114 kD
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CNKSR2/KSR2) Polyclonal Antibody, Unconjugated (TMAB-04487) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CNKSR2/KSR2) Polyclonal Antibody, Unconjugated (TMAB-04487) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CNKSR2

Human

CNKSR2

Connector enhancer of kinase suppressor of ras 2

May function as an adapter protein or regulator of Ras signaling pathways.