Anti-CLEC16A Polyclonal Antibody is a Rabbit antibody targeting CLEC16A. Anti-CLEC16A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human (predicted:Mouse,Rat,Pig,Cow)

1. Sample:
K562 (Human) Cell Lysate at 30 μg
Siha (Human) Cell Lysate at 30 μg
U-2OS (Human) Cell Lysate at 30 μg
U87MG (Human) Cell Lysate at 30 μg
Primary: Anti-CLEC16A (TMAB-04447) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 118 kD
Observed band size: 118 kD
2. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CLEC16A Polyclonal Antibody, Unconjugated (TMAB-04447) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody and DAB staining
3. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CLEC16A Polyclonal Antibody, Unconjugated (TMAB-04447) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody and DAB staining
4. Paraformaldehyde-fixed, paraffin embedded (human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30 min; Antibody incubation with (CLEC16A) Polyclonal Antibody, Unconjugated (TMAB-04447) at 1:500 overnight at 4℃, followed by a conjugated secondary for 20 minutes and DAB staining.
5. Sample: k562 (human) Cell Lysate at 40 μg
Primary: Anti-CLEC16A (TMAB-04447) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 118 kD
Observed band size: 118 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CLEC16A

Human

CLEC16A

Protein CLEC16A