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Anti-CLDN25 Polyclonal Antibody
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Catalog No. TMAB-04439
Anti-CLDN25 Polyclonal Antibody is a Rabbit antibody targeting CLDN25. Anti-CLDN25 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-CLDN25 Polyclonal Antibody
Anti-CLDN25 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-04439
Anti-CLDN25 Polyclonal Antibody is a Rabbit antibody targeting CLDN25. Anti-CLDN25 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Antigen Details
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| Description | Anti-CLDN25 Polyclonal Antibody is a Rabbit antibody targeting CLDN25. Anti-CLDN25 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Dog,Horse) |
| Verified Activity | 1. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-CLDN25 Polyclonal Antibody, Unconjugated (TMAB-04439) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Sample: 293T (human) cell Lysate at 40 μg Primary: Anti-CLDN25 (TMAB-04439) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25 kD Observed band size: 28 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell junction > tight junction. Cell membrane. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. |
| Immunogen | KLH conjugated synthetic peptide: human CLDN25 |
| Antigen Species | Human |
| Gene Name | CLDN25 |
| Gene ID | |
| Protein Name | Putative claudin-25 |
| Uniprot ID |
| Molecular Weight | Theoretical: 25 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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