Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF,FCM

Reactivity: Human,Mouse (predicted:Rat,Pig,Cow,Horse,Rabbit,Sheep)

IgG

Human,Mouse (predicted:Rat,Pig,Cow,Horse,Rabbit,Sheep)

1. Blank control: K562. Primary Antibody (green line): Rabbit Anti-Claudin 5 antibody (TMAB-00444)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Protein: lung (mouse) lysates at 45 μg;
Primary: Anti-Claudin 5 (TMAB-00444) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit Igg (bse-0295G-HRP) at 1: 5000;
ECL excitated the fluorescence;
Predicted band size:23 kDa
Observed band size:23 kDa
3. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Claudin 5) Polyclonal Antibody, Unconjugated (TMAB-00444) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB,IHC-P,IHC-Fr,IF,FCM

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2ug/Test

Polyclonal

KLH conjugated synthetic peptide: human Claudin 5

Human

7122

Tight Junctions