Anti-CLASP1 Antibody (1L48) is a Rabbit antibody targeting CLASP1. Anti-CLASP1 Antibody (1L48) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

IgG

1L48

Human,Mouse,Rat

1. Western blot analysis of HeLa cell lysate. Using CLASP1 (TMAB-04414) monoclonal antibody at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CLASP1 antibody (TMAB-04414, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:1000-2000; IHC-P: 1:100-200; IHC-Fr: 1:100-200; ICC/IF: 1:50-200; IF: 1:50-200; FCM: 1:50-100

Monoclonal

A synthesized peptide: human CLASP1

Human

CLASP1

CLIP-associating protein 1

Microtubule plus-end tracking protein that promotes thestabilization of dynamic microtubules. Involved in the nucleationof noncentrosomal microtubules originating from the trans-Golginetwork (TGN). Required for the polarization of the cytoplasmicmicrotubule arrays in migrating cells towards the leading edge ofthe cell. May act at the cell cortex to enhance the frequency ofrescue of depolymerizing microtubules by attaching their plus-endsto cortical platforms composed of ERC1 and PHLDB2. This corticalmicrotubule stabilizing activity is regulated at least in part byphosphatidylinositol 3-kinase signaling. Also performs a similarstabilizing function at the kinetochore which is essential for thebipolar alignment of chromosomes on the mitotic spindle.