Anti-CLACP Polyclonal Antibody is a Rabbit antibody targeting CLACP. Anti-CLACP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Chicken,Dog,Cow,Horse,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CLACP) Polyclonal Antibody, Unconjugated (TMAB-04413) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CLACP Polyclonal Antibody, Unconjugated (TMAB-04413) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Sample:
Heart (Mouse) Lysate at 40 μg
Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-CLACP (TMAB-04413) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 57 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CLACP

Human

COL25A1

Collagen alpha-1(XXV) chain

Antibodies against senile plaque (SP) amyloid obtained a clone 9D2, which labeled amyloid fibrils in SPs and reacted with approximately 50/100 kDa polypeptides in Alzheimer's diseased (AD) brains. The 9D2 antigen was identified as CLAC (collagen like Alzheimer amyloid plaque component), and its precursor as CLACP/collagen type XXV. Both secreted and membrane tethered forms of CLACP/collagen type XXV specifically bound to fibrillized Abeta, implicating these proteins in beta amyloidogenesis and neuronal degeneration in AD.