Anti-CK16 Antibody (8V670) is a Mouse antibody targeting CK16. Anti-CK16 Antibody (8V670) can be used in IHC-P,IHC-Fr,IF.

IgG

8V670

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CK16) Monoclonal Antibody, Unconjugated (ascites of TMAB-04389 8 h6) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human skin cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CK16) Monoclonal Antibody, Unconjugated (ascites of TMAB-04389 8 h6) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CK16) Monoclonal Antibody, Unconjugated (ascites of TMAB-04389 8 h6) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CK16) Monoclonal Antibody, Unconjugated (ascites of TMAB-04389 8 h6) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (rat skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CK16) Monoclonal Antibody, Unconjugated (ascites of TMAB-04389 8 h6) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CK16) Monoclonal Antibody, Unconjugated (ascites of TMAB-04389 8 h6) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CK16) Monoclonal Antibody, Unconjugated (TMAB-04389) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CK16) Monoclonal Antibody, Unconjugated (TMAB-04389) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody for 90 minutes, and DAPI for nuclei staining.
9. Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CK16 Monoclonal Antibody, Unconjugated (TMAB-04389) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CK16 Monoclonal Antibody, Unconjugated (TMAB-04389) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Rat Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CK16 Monoclonal Antibody, Unconjugated (TMAB-04389) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CK16 Monoclonal Antibody, Unconjugated (TMAB-04389) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CK16 Monoclonal Antibody, Unconjugated (TMAB-04389) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
14. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CK16 Monoclonal Antibody, Unconjugated (TMAB-04389) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
15. Paraformaldehyde-fixed, paraffin embedded Human Skin Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CK16 Monoclonal Antibody, Unconjugated (TMAB-04389) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
16. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CK16 Monoclonal Antibody, Unconjugated (TMAB-04389) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:200-800

Monoclonal

Recombinant Protein: human CK16 protein

Human

KRT16

Keratin, type I cytoskeletal 16