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Anti-CK12 Polyclonal Antibody

Catalog No. TMAB-04383 Copy Product Info
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Anti-CK12 Polyclonal Antibody is a Rabbit antibody targeting CK12. Anti-CK12 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-CK12 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-04383

Anti-CK12 Polyclonal Antibody is a Rabbit antibody targeting CK12. Anti-CK12 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-CK12 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2227-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-CK12 Polyclonal Antibody is a Rabbit antibody targeting CK12. Anti-CK12 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse (predicted:Human,Rat,Dog,Rabbit)
Verified Activity
Tissue/cell: mouse stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CK12 Polyclonal Antibody, Unconjugated (TMAB-04383) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Tissue SpecificityCornea specific.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundCytokeratin 12 is a member of the intermediate filament family of proteins and is a heterotetramer of two type I and two type II keratins. Keratin 3 is specifically expressed in the corneal epithelium with family member KRT12. Cytokeratin 12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Defects in KRT3 and KRT12 are a cause of Meesmann corneal dystrophy (MCD), an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Symptoms occur in adulthood and include rupture of the corneal microcysts that may lead to photophobia, contact lens intolerance and intermittent diminution of visual acuity. Defects in KRT12 are a cause of juvenile epithelial corneal dystrophy of Meesmann (MCD)
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CK12/Cytokeratin 12
Antigen Species
Human
Gene Name
KRT12
Gene ID
Protein Name
Keratin, type I cytoskeletal 12
Uniprot ID
Function
May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity (By similarity).
Chemical Properties
Molecular WeightTheoretical: 54 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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