Anti-CK-7 Antibody (6O60) is a Rabbit antibody targeting CK-7. Anti-CK-7 Antibody (6O60) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

6O60

Human

1. Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-04404
2. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1: 2000
Primary ab incubation condition: 2 hours at room temperature
Lysate: HeLa, MCF-7, A549
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 51 kDa
Observed MW: 50 kDa
3. Tissue: Human breast cancer
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for TMAB-04404
4. Tissue: Human ovarian carcinoma
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for TMAB-04404

WB: 1:500-2000; IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; ICC/IF: 1:50-200; IF: 1:200-1000

Monoclonal

KLH conjugated synthetic peptide: human CK-7

Human

KRT7

Keratin, type II cytoskeletal 7

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).