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Anti-CILP Polyclonal Antibody
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Catalog No. TMAB-00434
Alias CILP-1, CILP, Cartilage intermediate-layer protein, Cartilage intermediate layer protein 1
Anti-CILP Polyclonal Antibody is a Rabbit antibody targeting CILP. Anti-CILP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-CILP Polyclonal Antibody
Anti-CILP Polyclonal Antibody
😃Good
Catalog No. TMAB-00434Alias CILP-1, CILP, Cartilage intermediate-layer protein, Cartilage intermediate layer protein 1
Anti-CILP Polyclonal Antibody is a Rabbit antibody targeting CILP. Anti-CILP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CILP Polyclonal Antibody is a Rabbit antibody targeting CILP. Anti-CILP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | CILP-1, CILP, Cartilage intermediate-layer protein, Cartilage intermediate layer protein 1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Cow,Horse,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CILP) Polyclonal Antibody, Unconjugated (TMAB-00434) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: MCF-7 Cell (Human) Lysate at 30 μg Primary: Anti-CILP (TMAB-00434) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 132 kDa Observed band size: 132 kDa 3. Paraformaldehyde-fixed, paraffin embedded (mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CILP) Polyclonal Antibody, Unconjugated (TMAB-00434) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted > extracellular space > extracellular matrix. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Major alterations in the composition of the cartilage extracellular matrix occur in joint disease, such as osteoarthrosis. This gene encodes the cartilage intermediate layer protein (CILP), which increases in early osteoarthrosis cartilage. The encoded protein was thought to encode a protein precursor for two different proteins; an N-terminal CILP and a C-terminal homolog of NTPPHase, however, later studies identified no nucleotide pyrophosphatase phosphodiesterase (NPP) activity. The full-length and the N-terminal domain of this protein was shown to function as an IGF-1 antagonist. An allelic variant of this gene has been associated with lumbar disc disease. [provided by RefSeq, Sep 2010] |
| Immunogen | KLH conjugated synthetic peptide: human CILP |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Other ECM Proteins,Autoimmune |
| Molecular Weight | Theoretical: 132 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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