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Anti-CHRNA7 Polyclonal Antibody
😃Good
Catalog No. TMAB-00430
Anti-CHRNA7 Polyclonal Antibody is a Rabbit antibody targeting CHRNA7. Anti-CHRNA7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-CHRNA7 Polyclonal Antibody
Anti-CHRNA7 Polyclonal Antibody
😃Good
Catalog No. TMAB-00430
Anti-CHRNA7 Polyclonal Antibody is a Rabbit antibody targeting CHRNA7. Anti-CHRNA7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CHRNA7 Polyclonal Antibody is a Rabbit antibody targeting CHRNA7. Anti-CHRNA7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken) |
| Verified Activity | 1. Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-CHRNA7 Polyclonal Antibody, Unconjugated (TMAB-00430) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: human kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-CHRNA7 Polyclonal Antibody, Unconjugated (TMAB-00430) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus. 3. Sample: Lane 1: Human SH-SY5Y cell Lysates Lane 2: Human U-87 MG cell Lysates Lane 3: Human U251 cell Lysates Primary: Anti-CHRNA7 (TMAB-00430) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa 4. Sample: Lane 1: Cerebrum (Rat) Lysate at 40 μg Lane 2: Cerebrum (Mouse) Lysate at 40 μg Lane 3: Adrenal glands (Rat) Lysate at 40 μg Lane 4: Adrenal glands (Mouse) Lysate at 40 μg Lane 5: Placenta (Mouse) Lysate at 40 μg Lane 6: Lung (Mouse) Lysate at 40 μg Lane 7: Testis (Rat) Lysate at 40 μg Lane 8: HepG2 (Human) Cell Lysate at 30 μg Lane 9: SH-SY5Y (Human) Cell Lysate at 30 μg Primary: Anti-CHRNA7 (TMAB-00430) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56/50 kDa Observed band size: 48 kD |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The Nicotinic Acetylcholine Receptors are members of a superfamily of ligand gated ion channels that mediate fast signal transmission at synapses. These receptors are thought to be hetero pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C terminal extracellular region. The Nicotinic Acetylcholine Receptor alpha 7 forms a homo oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion conducting channel across the plasma membrane. |
| Immunogen | KLH conjugated synthetic peptide: human CHRNA7 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | nAch Receptors,Hypoxia,Response to hypoxia |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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