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Anti-CHRM3 Polyclonal Antibody

😃Good
Catalog No. TMAB-00429

Anti-CHRM3 Polyclonal Antibody is a Rabbit antibody targeting CHRM3. Anti-CHRM3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-CHRM3 Polyclonal Antibody

Anti-CHRM3 Polyclonal Antibody

😃Good
Catalog No. TMAB-00429
Anti-CHRM3 Polyclonal Antibody is a Rabbit antibody targeting CHRM3. Anti-CHRM3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$374 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Anti-CHRM3 Polyclonal Antibody is a Rabbit antibody targeting CHRM3. Anti-CHRM3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat)
Verified Activity
1. Sample: Mcf-7 Cell Lysate at 40 μg
Primary: Anti-Muscarinic Acetylcholine receptor (TMAB-00429) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa
2. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ChRM3/Acetylcholine receptor (M3) Polyclonal Antibody, Unconjugated (TMAB-00429) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. [provided by RefSeq].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human ChRM3
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Muscarinic Receptors,Acetylcholine
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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