Anti-CHRM3 Polyclonal Antibody is a Rabbit antibody targeting CHRM3. Anti-CHRM3 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human (predicted:Mouse,Rat)

1. Sample: Mcf-7 Cell Lysate at 40 μg
Primary: Anti-Muscarinic Acetylcholine receptor (TMAB-00429) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa
2. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ChRM3/Acetylcholine receptor (M3) Polyclonal Antibody, Unconjugated (TMAB-00429) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human ChRM3

Human

CHRM3

Muscarinic acetylcholine receptor M3

Muscarinic Receptors,Acetylcholine

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.