Anti-ChRM2 Polyclonal Antibody is a Rabbit antibody targeting ChRM2. Anti-ChRM2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Pig)

1. ample: Brain (Mouse) Lysate at 40 μg
Primary: Anti-ChRM2 (TMAB-04319) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
2. Tissue/cell: schwannoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-ChRM2 Polyclonal Antibody, Unconjugated (TMAB-04319) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Sample:
Lane 1: Mouse Spinal cord tissue lysates
Lane 2: Mouse Heart tissue lysates
Lane 3: Rat Spinal cord tissue lysates
Lane 4: Rat Heart tissue lysates
Primary: Anti-ChRM2 (TMAB-04319) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 47 kDa
4. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ChRM2) Polyclonal Antibody, Unconjugated (TMAB-04319) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human ChRM2

Human

CHRM2

Muscarinic acetylcholine receptor M2

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.