Anti-CHRM2 Antibody (3B102) is a Rabbit antibody targeting CHRM2. Anti-CHRM2 Antibody (3B102) can be used in WB,IHC-P,IHC-Fr,IP,IF.

IgG

3B102

Human,Mouse,Rat

1. 25 μg total protein per lane of various lysates (see on figure) probed with CHRM2 monoclonal antibody, unconjugated (TMAB-04320) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHRM2 Monoclonal Antibody, Unconjugated (TMAB-04320) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHRM2 Monoclonal Antibody, Unconjugated (TMAB-04320) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHRM2 Monoclonal Antibody, Unconjugated (TMAB-04320) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHRM2 Monoclonal Antibody, Unconjugated (TMAB-04320) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IP: 1:20-50; IF: 1:50-200

Monoclonal

A synthesized peptide: human CHRM2

Human

CHRM2

Muscarinic acetylcholine receptor M2

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.