Anti-CHGA Polyclonal Antibody 2 is a Rabbit antibody targeting CHGA. Anti-CHGA Polyclonal Antibody 2 can be used in IHC-P,WB,FCM,IF,IHC-Fr.

IgG

Mouse,Rat,Human

1. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CHGA) Polyclonal Antibody, Unconjugated (TMAB-04300) at 1: 600 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CHGA) Polyclonal Antibody, Unconjugated (TMAB-04300) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CHGA) Polyclonal Antibody, Unconjugated (TMAB-04300) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CHGA) Polyclonal Antibody, Unconjugated (TMAB-04300) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (human Gastric fundus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CHGA) Polyclonal Antibody, Unconjugated (TMAB-04300) at 1: 600 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CHGA) Polyclonal Antibody, Unconjugated (TMAB-04300) at 1:1000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CHGA) Polyclonal Antibody, Unconjugated (TMAB-04300) at 1: 600overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded (human pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CHGA) Polyclonal Antibody, Unconjugated (TMAB-04300) at 1:800overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded (Human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CHGA) Polyclonal Antibody, Unconjugated (TMAB-04300) at 1: 600 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded (human adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CHGA) Polyclonal Antibody, Unconjugated (TMAB-04300) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CHGA) Polyclonal Antibody, Unconjugated (TMAB-04300) at 1: 600 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CHGA) Polyclonal Antibody, Unconjugated (TMAB-04300) at 1: 600 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded (rat intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CHGA) Polyclonal Antibody, Unconjugated (TMAB-04300) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
14. Paraformaldehyde-fixed, paraffin embedded (mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CHGA) Polyclonal Antibody, Unconjugated (TMAB-04300) at 1:1000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
15. Sample:
Lane 1: Mouse Adrenal gland tissue lysates
Lane 2: Rat Adrenal gland tissue lysates
Lane 3: Recombinant mouse CHGA protein, C-His (HEK293)
Lane 4: Mouse NIH/3T3 cell lysates (negative control)
Primary: Anti-CHGA (TMAB-04300) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 33,76 kDa
16. Blank control (black line): SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-CHGA antibody (TMAB-04300)
Dilution: 2 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
17. Paraformaldehyde-fixed, paraffin embedded Human Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CHGA Polyclonal Antibody, Unconjugated (TMAB-04300) at 1: 600 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple), DAPI (blue) was used to stain the cell nuclei.
18. Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CHGA Polyclonal Antibody, Unconjugated (TMAB-04300) at 1: 600 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple), DAPI (blue) was used to stain the cell nuclei.
19. Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CHGA Polyclonal Antibody, Unconjugated (TMAB-04300) at 1: 600 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple), DAPI (blue) was used to stain the cell nuclei.
20. Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CHGA Polyclonal Antibody, Unconjugated (TMAB-04300) at 1: 600 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple), DAPI (blue) was used to stain the cell nuclei.

IHC-P: 1:500-1000; WB: 1:500-2000; FCM: 2μg/Test; IF: 1:500-1000; IHC-Fr: 1:500-1000

Polyclonal

Recombinant Protein: mouse CHGA protein

Mouse

CHGA

Chromogranin-A

Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.