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Anti-CHD1L Antibody (7L400) is a Rabbit antibody targeting CHD1L. Anti-CHD1L Antibody (7L400) can be used in WB,IHC,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $298 | 7-10 days | |
100 μL | $498 | 7-10 days |
Description | Anti-CHD1L Antibody (7L400) is a Rabbit antibody targeting CHD1L. Anti-CHD1L Antibody (7L400) can be used in WB,IHC,FCM. |
Synonyms | Chromodomain-helicase-DNA-binding protein 1-like, CHD1L, Amplified in liver cancer protein 1, ALC1 |
Ig Type | IgG |
Reactivity | Human,Mouse |
Verified Activity | 1. Western blot analysis of CHD1L on HepG2 cell using anti-CHD1L antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CHD1L antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. 3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CHD1L antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. 4. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-CHD1L antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. 5. Flow cytometric analysis of HepG2 cells with CHD1L antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Application | |
Recommended Dose | WB: 1:500-1000; IHC: 1:50-100; FCM: 1:50-100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | CHD1L (Chromodomain helicase DNA-binding protein 1-like) is a 897 amino acid protein encoded by the human gene CHD1L. The CHD family of proteins are ATP-dependent chromatin remodeling enzymes, which combine chromodomains, with SWI2/SNF2 ATPase/helicase motifs and DNA-binding capability. Chromodomains are protein regions of about 40-50 amino acid residues found in proteins associated with chromatin remodeling and manipulation. The domain is highly conserved among both plants and animals and is found in a large variety of proteins from many genomes. CHD1L and CHD1 share most homology within two internal helicase domains. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: human CHD1L aa 600-800 |
Antigen Species | human |
Uniprot ID |
Molecular Weight | Theoretical: 101/90 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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