Anti-CHAT Polyclonal Antibody is a Rabbit antibody targeting CHAT. Anti-CHAT Polyclonal Antibody can be used in WB,FCM.

IgG

Human

1. Blank control: SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-ChAT antibody (TMAB-00423)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Sample:
Lane 1: Human SH-SY5Y cell lysates
Lane 2: Human U251 cell lysates
Primary: Anti-ChAT (TMAB-00423) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 82 kDa
Observed band size: 72 kDa

WB,FCM

WB: 1:500-2000; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human ChAT

Human

Soma marker,Acetylcholine