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Anti-CHAT Antibody (3G910)
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Catalog No. TMAC-00850
Anti-CHAT Antibody (3G910) is a Rabbit antibody targeting CHAT. Anti-CHAT Antibody (3G910) can be used in WB,IP,ICC/IF,IHC,FCM.
Anti-CHAT Antibody (3G910)
Anti-CHAT Antibody (3G910)
😃Good
Catalog No. TMAC-00850
Anti-CHAT Antibody (3G910) is a Rabbit antibody targeting CHAT. Anti-CHAT Antibody (3G910) can be used in WB,IP,ICC/IF,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-CHAT Antibody (3G910) is a Rabbit antibody targeting CHAT. Anti-CHAT Antibody (3G910) can be used in WB,IP,ICC/IF,IHC,FCM. |
| Ig Type | IgG |
| Clone | 3G910 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- Choline Acetyltransferase antibody. Counter stained with hematoxylin. 2. Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti- Choline Acetyltransferase antibody. Counter stained with hematoxylin. 3. ICC staining ChoLine Acetyltransferase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Choline Acetyltransferase in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Choline Acetyltransferase in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of N2A cells with Choline Acetyltransferase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. 7. Lane1 Mouse brain tissue lysate Use the Antibody at 1:2K dilution for 1 hour at room temperature. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:100-200; ICC/IF: 1:50-200; IP: 1:20-50; FCM: 1:20-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| Research Background | Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. |
| Conjucates | Unconjugated |
| Immunogen | A synthesized peptide: human CHAT |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 82 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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