Anti-CHAC1 Polyclonal Antibody is a Rabbit antibody targeting CHAC1. Anti-CHAC1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Dog,Pig,Cow,Rabbit)

1. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Cerebrum (Rat) Lysate at 40 μg
Primary: Anti-CHAC1 (TMAB-04280) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 29 kD
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CHAC1 Polyclonal Antibody, Unconjugated (TMAB-04280) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CHAC1) Polyclonal Antibody, Unconjugated (TMAB-04280) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CHAC1

Human

CHAC1

Glutathione-specific gamma-glutamylcyclotransferase 1

Pro-apoptotic component of the unfolded protein response pathway. May mediate the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade.