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Anti-CH25H Polyclonal Antibody

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Catalog No. TMAB-00421

Anti-CH25H Polyclonal Antibody is a Rabbit antibody targeting CH25H. Anti-CH25H Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-CH25H Polyclonal Antibody

Anti-CH25H Polyclonal Antibody

😃Good
Catalog No. TMAB-00421
Anti-CH25H Polyclonal Antibody is a Rabbit antibody targeting CH25H. Anti-CH25H Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$372 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Anti-CH25H Polyclonal Antibody is a Rabbit antibody targeting CH25H. Anti-CH25H Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human
Verified Activity
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CH25H) Polyclonal Antibody, Unconjugated (TMAB-00421) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
IHC-P,IHC-Fr,IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationEndoplasmic reticulum membrane
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundCH25H (Cholesterol 25-hydroxylase), also known as h25OH, is a 272 amino acid endoplasmic membrane protein that belongs to the sterol desaturase family. CH25H contains clusters of histidine residues essential for catalytic activity and is involved in cholesterol and lipid metabolism. CH25H catalyzes the formation of 25-hydroxycholesterol from cholesterol leading to the repression of cholesterol biosynthetic enzymes. CH25H regulates lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. CH25H utilizes diiron cofactors to catalyze the hydroxylation of hydrophobic substrates.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CH25H
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Metabolism of lipids and lipoproteins,Cholesterol Metabolism,Oxidative Stress,Cholesterol Metabolism,Lipid metabolism,Oxidative stress
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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