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Anti-CH25H Polyclonal Antibody
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Catalog No. TMAB-00421
Alias h25OH, EC 1.14.99.38, Cholesterol 25 monooxygenase, Cholesterol 25 hydroxylase, CH25H, C25H
Anti-CH25H Polyclonal Antibody is a Rabbit antibody targeting CH25H. Anti-CH25H Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-CH25H Polyclonal Antibody
Anti-CH25H Polyclonal Antibody
😃Good
Catalog No. TMAB-00421Alias h25OH, EC 1.14.99.38, Cholesterol 25 monooxygenase, Cholesterol 25 hydroxylase, CH25H, C25H
Anti-CH25H Polyclonal Antibody is a Rabbit antibody targeting CH25H. Anti-CH25H Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CH25H Polyclonal Antibody is a Rabbit antibody targeting CH25H. Anti-CH25H Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Alias | h25OH, EC 1.14.99.38, Cholesterol 25 monooxygenase, Cholesterol 25 hydroxylase, CH25H, C25H |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CH25H) Polyclonal Antibody, Unconjugated (TMAB-00421) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Endoplasmic reticulum membrane |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | CH25H (Cholesterol 25-hydroxylase), also known as h25OH, is a 272 amino acid endoplasmic membrane protein that belongs to the sterol desaturase family. CH25H contains clusters of histidine residues essential for catalytic activity and is involved in cholesterol and lipid metabolism. CH25H catalyzes the formation of 25-hydroxycholesterol from cholesterol leading to the repression of cholesterol biosynthetic enzymes. CH25H regulates lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. CH25H utilizes diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. |
| Immunogen | KLH conjugated synthetic peptide: human CH25H |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Metabolism of lipids and lipoproteins,Cholesterol Metabolism,Oxidative Stress,Cholesterol Metabolism,Lipid metabolism,Oxidative stress |
| Molecular Weight | Theoretical: 32 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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