Anti-CENPC1 Antibody (8J653) is a Rabbit antibody targeting CENPC1. Anti-CENPC1 Antibody (8J653) can be used in WB,ICC/IF.

IgG

8J653

Human,Mouse

1. 25 μg total protein per lane of various lysates (see on figure) probed with CENPC1 monoclonal antibody, unconjugated (TMAB-04225) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. 4% Paraformaldehyde-fixed K562 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (CENPC1) monoclonal Antibody, unconjugated (TMAB-04225) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

WB: 1:500-2000; ICC/IF: 1:50-200

Monoclonal

A synthesized peptide: human CENPC

Human

CENPC

Centromere protein C

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPC recruits DNA methylation and DNMT3B to both centromeric and pericentromeric satellite repeats and regulates the histone code in these regions.