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Anti-CEBP Beta Polyclonal Antibody 2 is a Rabbit antibody targeting CEBP Beta. Anti-CEBP Beta Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
| Description | Anti-CEBP Beta Polyclonal Antibody 2 is a Rabbit antibody targeting CEBP Beta. Anti-CEBP Beta Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CEBP Beta) Polyclonal Antibody, Unconjugated (TMAB-04208) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: Lane 1: Liver (Mouse) Tissue Lysate at 40 μg Lane 2: Brl-3a (Human) Cell Lysate at 30 μg Lane 3: U937 (Human) Cell Lysate at 30 μg Primary: Anti-CEBP Beta (TMAB-04208) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:400-800; IHC-Fr: 1:400-800; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. |
| Tissue Specificity | Expressed at low levels in the lung, kidney and spleen. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene. [provided by RefSeq]. |
| Immunogen | KLH conjugated synthetic peptide: human CEBP Beta |
| Antigen Species | Human |
| Gene Name | CEBPB |
| Gene ID | |
| Protein Name | CCAAT/enhancer-binding protein beta |
| Uniprot ID | |
| Function | Involved in interleukin-6 signal transduction, including the transcriptional activation of acute-phase genes. Functions in brown adipose tissue (BAT) differentiation. |
| Molecular Weight | Theoretical: 36 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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