Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

Anti-CEBP beta Polyclonal Antibody

😃Good
Catalog No. TMAB-00416

Anti-CEBP beta Polyclonal Antibody is a Rabbit antibody targeting CEBP beta. Anti-CEBP beta Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-CEBP beta Polyclonal Antibody

Anti-CEBP beta Polyclonal Antibody

😃Good
Catalog No. TMAB-00416
Anti-CEBP beta Polyclonal Antibody is a Rabbit antibody targeting CEBP beta. Anti-CEBP beta Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$374 7-10 days
200 μL$529 7-10 days
Add to Cart
Add to Quotation
Bulk & Custom
Questions
View More
Contact us for more batch information
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity
Description
Anti-CEBP beta Polyclonal Antibody is a Rabbit antibody targeting CEBP beta. Anti-CEBP beta Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Chicken,Pig,Cow,Rabbit,Sheep)
Verified Activity
1. Sample:
HepG2 (Human) Cell Lysate at 40 μg
Primary: Anti-CEBP Beta (TMAB-00416) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
2. Sample:
Lung (Mouse) Lysate at 40 μg
Primary: Anti-CEBP Beta (TMAB-00416) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
3. Blank control (blue line): Hela
Primary Antibody (green line): Rabbit Anti-CEBP Beta antibody (TMAB-00416)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1 μg/test.
Protocol
The cells were fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature.
4. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CEBP Beta) Polyclonal Antibody, Unconjugated (TMAB-00416) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1µg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene. [provided by RefSeq].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CEBP Beta
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Diabetes associated,Transcription factors,Other factors,Transcription Factors
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

Sci Citations

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: buy Anti-CEBP beta Polyclonal Antibody | purchase Anti-CEBP beta Polyclonal Antibody | Anti-CEBP beta Polyclonal Antibody cost | order Anti-CEBP beta Polyclonal Antibody