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Anti-CEACAM5 Antibody (2B683)

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Catalog No. TMAB-04204

Anti-CEACAM5 Antibody (2B683) is a Rabbit antibody targeting CEACAM5. Anti-CEACAM5 Antibody (2B683) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-CEACAM5 Antibody (2B683)

Anti-CEACAM5 Antibody (2B683)

Copy Product Info
😃Good
Catalog No. TMAB-04204
Anti-CEACAM5 Antibody (2B683) is a Rabbit antibody targeting CEACAM5. Anti-CEACAM5 Antibody (2B683) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1517-10 days7-10 days
50 μL$2637-10 days7-10 days
100 μL$4717-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-CEACAM5 Antibody (2B683) is a Rabbit antibody targeting CEACAM5. Anti-CEACAM5 Antibody (2B683) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Ig Type
IgG
Clone
2B683
Reactivity
Human
Verified Activity
1. 25 μg total protein per lane of various lysates (see on figure) probed with CEACAM5 monoclonal antibody, unconjugated (TMAB-04204) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CEACAM5 Monoclonal Antibody, Unconjugated (TMAB-04204) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CEACAM5 Monoclonal Antibody, Unconjugated (TMAB-04204) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CEACAM5 Monoclonal Antibody, Unconjugated (TMAB-04204) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CEACAM5 Monoclonal Antibody, Unconjugated (TMAB-04204) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-200; IHC-Fr: 1:100-200; ICC/IF: 1:50-200; IF: 1:100-200
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Lipid-anchor, GPI-anchor
Tissue SpecificityFound in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundCell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6.
Antigen Details
Immunogen
A synthesized peptide: human CEACAM5
Antigen Species
Human
Gene Name
CEACAM5
Gene ID
Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 6
Uniprot ID
Function
Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.
Chemical Properties
Molecular WeightTheoretical: 77 kDa. Actual: 180-220 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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