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Anti-CEACAM1 Antibody (4J334)
😃Good
Catalog No. TMAY-03358
Alias CEACAM‑1, CD66a, carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein), BGPI, BGP1, BGP
Anti-CEACAM1 Antibody (4J334) is a Rabbit antibody targeting CEACAM1. Anti-CEACAM1 Antibody (4J334) can be used in FCM,ICC/IF.
Anti-CEACAM1 Antibody (4J334)
Anti-CEACAM1 Antibody (4J334)
😃Good
Catalog No. TMAY-03358Alias CEACAM‑1, CD66a, carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein), BGPI, BGP1, BGP
Anti-CEACAM1 Antibody (4J334) is a Rabbit antibody targeting CEACAM1. Anti-CEACAM1 Antibody (4J334) can be used in FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $93 | 7-10 days | |
| 100 μL | $269 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: FCM, ICC/IF Reactivity: Mouse |
| Alias | CEACAM‑1, CD66a, carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein), BGPI, BGP1, BGP |
| Ig Type | Rabbit IgG |
| Clone | 4J334 |
| Reactivity | Mouse |
| Verified Activity | 1. Immunofluorescence staining of mouse CEACAM1 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CEACAM1 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). 2. Flow cytometric analysis of Mouse CEACAM1(CD66a) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse CEACAM1(CD66a), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CEACAM1 / CD66a (rM CEACAM1 / CD66a; TMPY-01676; NP_001034274.1; Met1-Gly428). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The carcinoembryonic-antigen-related cell-adhesion molecule (CEACAM) family of proteins has been implicated in various intercellular-adhesion and intracellular-signalling-mediated effects that govern the growth and differentiation of normal and cancerous cells. CEACAM1, also known as biliary glycoprotein I (BGP I) and CD66a, is a member of the carcinoembryonic antigen (CEA) gene family which belongs to the immunoglobulin superfamily. The highly glycosylated CEACAM1 contains one N-terminal V-type Ig-like domain and three C2-type Ig-like domains within its ECD, and one ITIM motif and a calmodulin binding site in the cytoplasmic region. CEACAM1 is a surface glycoprotein expressed on various blood cells, epithelial cells, and vascular cells. It was described as an adhesion molecule mediating cell adhesion via both homophilic and heterophilic manners, and was detected on leukocytes, epithelia, and endothelia. Studies have revealed that CEACAM1 performs actions in multiple cellular processes including tissue differentiation, angiogenesis, apoptosis, metastasis, as well as the modulation of innate and adaptive immune responses.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint Detection: IHC AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy |
| Conjucates | Unconjugated |
| Others Formats | APC/PerCP/FITC/PE |
| Antibody Types Available | 5 |
| Immunogen | Recombinant Protein: Mouse CEACAM1 / CD66a Protein (TMPY-01676) |
| Antigen Species | Mouse |
| Biology Area | Cancer Drug Targets, ITIM/ITAM Immunoreceptors and Related Molecules |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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