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Anti-CEA Antibody (1A582)

Catalog No. TMAB-04197 Copy Product Info
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Anti-CEA Antibody (1A582) is a Mouse antibody targeting CEA. Anti-CEA Antibody (1A582) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-CEA Antibody (1A582)

Copy Product Info
🥰Excellent
Catalog No. TMAB-04197

Anti-CEA Antibody (1A582) is a Mouse antibody targeting CEA. Anti-CEA Antibody (1A582) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-CEA Antibody
(1A582)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2227-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5277-10 days7-10 days
In stock · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-CEA Antibody (1A582) is a Mouse antibody targeting CEA. Anti-CEA Antibody (1A582) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Clone
1A582
Reactivity
Human
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CEA) Monoclonal Antibody, Unconjugated (TMAB-04197) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Sample:
Lane 1: Human LOVO cell lysates
Lane 2: Recombinant human CEACAM5 protein, C-His
Primary: Anti-CEACAM5 (TMAB-04197) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 200, 140 kDa
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:400-800; IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationCell membrane; Lipid-anchor, GPI-anchor
Tissue SpecificityFound in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
ConstructionHybridoma Monoclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundCEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glyco-protein (PSG 1-PSG 11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/ CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CEA/CD66e/CEACAM5
Antigen Species
Human
Gene Name
CEACAM5
Gene ID
Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 6
Uniprot ID
Function
Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.
Chemical Properties
Molecular WeightTheoretical: 150-200 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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