Anti-CDKN2A/p16-INK4a Antibody (7K769) is a Rabbit antibody targeting CDKN2A/p16-INK4a. Anti-CDKN2A/p16-INK4a Antibody (7K769) can be used in IHC-P,IHC-Fr,IF,WB,ICC/IF.

IgG

7K769

Human

1. 25 μg total protein per lane of various lysates (see on figure) probed with CDKN2A monoclonal antibody, unconjugated (TMAB-04183) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDKN2A Monoclonal Antibody, Unconjugated (TMAB-04183) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDKN2A Monoclonal Antibody, Unconjugated (TMAB-04183) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Ovarian mucinous carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDKN2A Monoclonal Antibody, Unconjugated (TMAB-04183) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDKN2A Monoclonal Antibody, Unconjugated (TMAB-04183) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (CDKN2A) monoclonal Antibody, unconjugated (TMAB-04183) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; WB: 1:500-2000; ICC/IF: 1:50-200

Monoclonal

A synthesized peptide: human CDKN2A

Human

Cdkn2a

Tumor suppressor ARF

Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.