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Anti-CDKN1B Antibody (9B660) (Synonyms: p27Kip1, KIP1, Cyclin-Dependent Kinase Inhibitor p27, Cyclin-Dependen...)

Catalog No. TMAC-02947 Copy Product Info
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Anti-CDKN1B Antibody (9B660) is a Rabbit antibody targeting CDKN1B. Anti-CDKN1B Antibody (9B660) can be used in FCM,ICC/IF,IHC,IP,WB.

Anti-CDKN1B Antibody (9B660)

Copy Product Info
🥰Excellent
Catalog No. TMAC-02947
Synonyms p27Kip1, KIP1, Cyclin-Dependent Kinase Inhibitor p27, Cyclin-Dependent Kinase Inhibitor 1B, CDKN1B

Anti-CDKN1B Antibody (9B660) is a Rabbit antibody targeting CDKN1B. Anti-CDKN1B Antibody (9B660) can be used in FCM,ICC/IF,IHC,IP,WB.

Anti-CDKN1B Antibody
(9B660)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2977-10 days7-10 days
100 μL$4987-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-CDKN1B Antibody (9B660) is a Rabbit antibody targeting CDKN1B. Anti-CDKN1B Antibody (9B660) can be used in FCM,ICC/IF,IHC,IP,WB.
Synonymsp27Kip1, KIP1, Cyclin-Dependent Kinase Inhibitor p27, Cyclin-Dependent Kinase Inhibitor 1B, CDKN1B
Ig Type
IgG
Clone
9B660
Reactivity
Human,Rat
Verified Activity
1. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-p27 KIP 1 antibody. Counter stained with hematoxylin.
2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-p27 KIP 1 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-p27 KIP 1 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-p27 KIP 1 antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-p27 KIP 1 antibody. Counter stained with hematoxylin.
6. ICC staining p27 KIP 1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining p27 KIP 1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. Flow cytometric analysis of Hela cells with p27 KIP 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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Application
Recommended Dose
WB: 1:1000; IHC: 1:100-500; ICC/IF: 1:100-500; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundCell cycle progression is regulated by a series of cyclin-dependent kinases consisting of catalytic subunits, designated Cdks, as well as activating subunits, designated cyclins. Orderly progression through the cell cycle requires the activation and inactivation of different cyclin-Cdks at appropriate times. A series of proteins has recently been described that function as "mitotic inhibitors." These include p21, the levels of which are elevated upon DNA damage in G1 in a p53-dependent manner; p16; and a more recently described p16-related inhibitor designated p15. A p21-related protein, p27, has been described as a negative regulator of G1 progression and speculated to function as a possible mediator of TGFβ-induced G1 arrest. p27 interacts strongly with D-type cyclins and Cdk4 in vitro and, to a lesser extent, with cyclin E and Cdk2.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 27 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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