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Anti-CDKN1B Antibody (9B660)
😃Good
Catalog No. TMAC-02947
Alias p27Kip1, KIP1, Cyclin-Dependent Kinase Inhibitor p27, Cyclin-Dependent Kinase Inhibitor 1B, CDKN1B
Anti-CDKN1B Antibody (9B660) is a Rabbit antibody targeting CDKN1B. Anti-CDKN1B Antibody (9B660) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-CDKN1B Antibody (9B660)
Anti-CDKN1B Antibody (9B660)
😃Good
Catalog No. TMAC-02947Alias p27Kip1, KIP1, Cyclin-Dependent Kinase Inhibitor p27, Cyclin-Dependent Kinase Inhibitor 1B, CDKN1B
Anti-CDKN1B Antibody (9B660) is a Rabbit antibody targeting CDKN1B. Anti-CDKN1B Antibody (9B660) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-CDKN1B Antibody (9B660) is a Rabbit antibody targeting CDKN1B. Anti-CDKN1B Antibody (9B660) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | p27Kip1, KIP1, Cyclin-Dependent Kinase Inhibitor p27, Cyclin-Dependent Kinase Inhibitor 1B, CDKN1B |
| Ig Type | IgG |
| Reactivity | Human,Rat |
| Verified Activity | 1. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-p27 KIP 1 antibody. Counter stained with hematoxylin. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-p27 KIP 1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-p27 KIP 1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-p27 KIP 1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-p27 KIP 1 antibody. Counter stained with hematoxylin. 6. ICC staining p27 KIP 1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining p27 KIP 1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Hela cells with p27 KIP 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000; IHC: 1:100-500; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Cell cycle progression is regulated by a series of cyclin-dependent kinases consisting of catalytic subunits, designated Cdks, as well as activating subunits, designated cyclins. Orderly progression through the cell cycle requires the activation and inactivation of different cyclin-Cdks at appropriate times. A series of proteins has recently been described that function as "mitotic inhibitors." These include p21, the levels of which are elevated upon DNA damage in G1 in a p53-dependent manner; p16; and a more recently described p16-related inhibitor designated p15. A p21-related protein, p27, has been described as a negative regulator of G1 progression and speculated to function as a possible mediator of TGFβ-induced G1 arrest. p27 interacts strongly with D-type cyclins and Cdk4 in vitro and, to a lesser extent, with cyclin E and Cdk2. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 27 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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