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Anti-CDK6 Antibody (5T828)
😃Good
Catalog No. TMAC-00797
Alias Serine/threonine-protein kinase PLSTIRE, EC 2.7.11.22, Cyclin-dependent kinase 6, Cell division protein kinase 6, CDKN6, CDK 6
Anti-CDK6 Antibody (5T828) is a Rabbit antibody targeting CDK6. Anti-CDK6 Antibody (5T828) can be used in WB,ICC/IF,IHC,FCM.
Anti-CDK6 Antibody (5T828)
Anti-CDK6 Antibody (5T828)
😃Good
Catalog No. TMAC-00797Alias Serine/threonine-protein kinase PLSTIRE, EC 2.7.11.22, Cyclin-dependent kinase 6, Cell division protein kinase 6, CDKN6, CDK 6
Anti-CDK6 Antibody (5T828) is a Rabbit antibody targeting CDK6. Anti-CDK6 Antibody (5T828) can be used in WB,ICC/IF,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-CDK6 Antibody (5T828) is a Rabbit antibody targeting CDK6. Anti-CDK6 Antibody (5T828) can be used in WB,ICC/IF,IHC,FCM. |
| Synonyms | Serine/threonine-protein kinase PLSTIRE, EC 2.7.11.22, Cyclin-dependent kinase 6, Cell division protein kinase 6, CDKN6, CDK 6 |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of Cdk6 on different lysates using anti-Cdk6 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat, Lane 2: K562, Lane 3: Hela. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cdk6 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cdk6 antibody. Counter stained with hematoxylin. 4. ICC staining Cdk6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Cdk6 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Cdk6 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of Hela cells with Cdk6 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression. Another family of proteins, Cdk inhibitors, also plays a role in regulating the cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1-PCTAIRE-3, PITALRE and PITSLRE. Cdk6 is known to associate with cyclins D1, D2 and D3 and to be involved with the G1/S transition of the cell cycle. Multiple inhibitors of Cdk6 have been identified, including p18 and p19. These inhibitors bind to both free and complexed Cdk6, and they inhibit the activity of the cyclin D-bound Cdk6. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 37 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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